{"id":1527,"date":"2022-12-15T10:13:35","date_gmt":"2022-12-15T10:13:35","guid":{"rendered":"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1527"},"modified":"2022-12-15T18:06:58","modified_gmt":"2022-12-15T18:06:58","slug":"ati2","status":"publish","type":"page","link":"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1527","title":{"rendered":"ATI2"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1527\" class=\"elementor elementor-1527\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a60c1ce elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"a60c1ce\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c0a5566\" data-id=\"c0a5566\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d29922a elementor-widget elementor-widget-heading\" data-id=\"d29922a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.0 - 06-12-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">ATI2 - Preparation and functional evaluation of collagen oligopeptide-rich hydrolysates<br><\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a079a54 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"a079a54\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d3fe95b\" data-id=\"d3fe95b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a7f74d0 elementor-widget elementor-widget-heading\" data-id=\"a7f74d0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Objectives<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3119c5a elementor-widget elementor-widget-text-editor\" data-id=\"3119c5a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.0 - 06-12-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><strong><em>O3<\/em><\/strong> &#8211; Obtain collagen oligopeptides-rich extracted from P. physalis using cold-adapted proteases from psychrophiles bacteria isolated from deep-sea.<\/p><p><em><strong>O4<\/strong><\/em> \u2013 Characterization of the collagen oligopeptides-rich in terms of potential to be used in medic or dermo cosmetic applications.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bfa2f6e elementor-widget elementor-widget-heading\" data-id=\"bfa2f6e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI2<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b5a4ed elementor-widget elementor-widget-text-editor\" data-id=\"7b5a4ed\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>This activity has two main goals. The first goal is to obtain collagen oligopeptides-rich extracted from <em>P. physalis <\/em>using cold- adapted proteases from psychrophiles bacteria isolated from deep-sea. The second goal is to characterize the obtain collagen oligopeptides-rich in terms of its bioactivity and potential to be used in medic or dermo cosmetic applications.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-90626f9 elementor-widget elementor-widget-heading\" data-id=\"90626f9\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 2.1 - Production of collagen oligopeptides<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-96fbcc0 elementor-widget elementor-widget-text-editor\" data-id=\"96fbcc0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"font-size: 16px;\">Hydrolysed collagen is an advanced version of collagen that has high-value applications because of its high solubility, excellent dispersion and functional activities. The CBA research group from the University of Azores has a collection of psychrophiles bacteria isolated from deep-sea sediments, which can efficiently hydrolyse collagen into peptides. To achieve the first goal, several steps are required: i) optimization of the fermentation conditions for extracellular protease production; ii) hydrolyses of the collagen from P. physalis by the microbial extracellular proteases, and; iii) hydrolyses of the collagen using commercial exogenous enzymes. The profile of produced hydrolysates will be analysed by MALDI-TOF in order to select those with the highest content of low-molecular-weight oligopeptides (4 \u2013 10 aa residues), which are those that are easily absorbed. The hydrolysates will be shared for biological activities with UM (T2.2). This task will be implemented by UA\u00c7.<\/span><br><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fa98cd4 elementor-widget elementor-widget-heading\" data-id=\"fa98cd4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 2.2 - Assessment of the peptide's bioactivity<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0bac872 elementor-widget elementor-widget-text-editor\" data-id=\"0bac872\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Prior to the assessment of the hydrolysates bioactivity its toxicity will be evaluated using different cells lines, such as fibroblasts (ATCC-CRL2524), primary epidermal keratinocytes (PCS-200-011) and epidermal melanocytes. Lactate dehydrogenase will be used to measure the induce toxicity of the peptides. The ability of this new set of collagen peptides in cellular proliferation will be evaluated by MTT. While proliferation is an important parameter to be addressed, the influence on the cell cycle is of upmost importance. The cell cycle will be analysed by flow cytometry. It is expected that peptides elicit a positive response on skin cells promoting the production of collagen and keratin, therefore the gene expression encoding for it and the production of these proteins will be assessed by RT-PCR and ELISA, respectively. In the case of a medical application for these peptides, like chronic wounds, it is necessary to evaluate the potential of these peptides to reduce the inflammatory stage of wound healing. So, the inflammatory response induced by the peptides will be evaluate upon contact with macrophages pre-activated and non-activated. The production of inflammatory and anti-inflammatory cytokines will be evaluated using ELISA kits. For chronic wounds applications it also important to evaluate the capacity of these peptides to induce cellular migration, for that a wound assay will be performed. On the other hand, if applying to the cosmetic industry, it is necessary to evaluate the antioxidant activity potential, as one of the major cause aging is the production reactive oxygen species. Newer in vitro models are available to have a depth understanding, in terms of layer morphology and cytokine profile, of the influence of different molecules on skin regeneration, like 3D skin model. These models comprise primary fibroblasts, hTERT immortalized MSCs and BJ-5tA (ATCC). The proper biochemical function of the skin is guaranteed by an adequate blood supply and if untypical angiogenesis is detected, it means that molecules have a negative effect on skin regeneration which can leads to fibrosis and scaring. Thereby, the influence of the peptides in angiogenesis will be evaluated using chick chorioallantoic membrane assay. At the end of this task the bioactivity potential of the peptides will be fully characterized. The transcriptional prolife of the epidermal cells will be determined upon contact with the peptides or assemble of peptides with highest bioactivity as described in task T2.3. This task is the responsibility of the UM.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1351908 elementor-widget elementor-widget-heading\" data-id=\"1351908\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 2.3 - Transcriptional Profiling of Epidermal cells<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c92e465 elementor-widget elementor-widget-text-editor\" data-id=\"c92e465\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>In this task, a transcriptomic approach will be used to highlight the mode of action of hydrolysates with positive effect immune-modulation, cell differentiation and migration, selected in T2.2. The gene expression analysis can provide relevant information about the altered expression of genes encoding the critical biological processes responsible for inflammation and skin regeneration, such as extracellular matrix organization, collagen fibril organization, and cell adhesion. The level expression of anti-inflammatory interleukins (e.g. IL-1, IL-8), vascular endothelial growth factors and matrix metalloproteinase (e.g. MMP-9 and MMP-13) will be evaluated by RNAseq. This task will produce data valuable for demonstrating compliance with applicable requirements in cosmetics. This task is the responsibility of the UA\u00e7.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-00a502d elementor-widget elementor-widget-heading\" data-id=\"00a502d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Participants<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4d80c56 elementor-widget elementor-widget-text-editor\" data-id=\"4d80c56\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><span style=\"font-size: 16px;\">Mesosystem S.A.<\/span><\/li><li><span style=\"font-size: 16px;\">Universidade do Minho<\/span><\/li><li><span style=\"font-size: 16px;\">UA\u00e7<\/span><\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8287b46 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"8287b46\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-af4bafc\" data-id=\"af4bafc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4b0a8c3 wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"4b0a8c3\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1436\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI1<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-9c633b8\" data-id=\"9c633b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5b9f15e wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"5b9f15e\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1538\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI3<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-f701584\" data-id=\"f701584\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f9dcf7e wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"f9dcf7e\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1552\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI4<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-8b3c83a\" data-id=\"8b3c83a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-18dcd08 wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"18dcd08\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1560\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI5<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-14caf53\" data-id=\"14caf53\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d077bd wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"3d077bd\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1568\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI6<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c3b5737 elementor-section-height-min-height elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-items-middle wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"c3b5737\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-137aa967 elementor-hidden-tablet elementor-hidden-mobile\" data-id=\"137aa967\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-abd5fbc elementor-widget elementor-widget-heading\" data-id=\"abd5fbc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Join our mailing 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class=\"wpr-button-text\">Subscribe now<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e9a91f9 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"e9a91f9\" data-element_type=\"section\" id=\"contact-us\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-13a6ebb0\" data-id=\"13a6ebb0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap 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*\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon 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100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b99548c elementor-widget elementor-widget-text-editor\" data-id=\"7b99548c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>More important than what we have to say about ourselves, is what the customers have to say about us.<\/p><p>So let us learn and contact us!<\/p><p>We create possibilites for the connnected world &#8211; Mesosystem S.A.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-5813cd5a\" data-id=\"5813cd5a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-17174383 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"17174383\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-af1903c\" data-id=\"af1903c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7e2d7be1 elementor-widget elementor-widget-heading\" data-id=\"7e2d7be1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Email<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3691c7df elementor-widget elementor-widget-text-editor\" data-id=\"3691c7df\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>info@mesosystem.com<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2c008cb5 elementor-widget elementor-widget-heading\" data-id=\"2c008cb5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Phone<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-64d1386e elementor-widget elementor-widget-text-editor\" data-id=\"64d1386e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Telephone (+351) 227 347 125 (Call to a national fixed network)<\/p><p>Mobile (+351) 924 188 704 (Call to a national mobile network)<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a9afaba elementor-widget elementor-widget-heading\" data-id=\"6a9afaba\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Location<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8810b05 elementor-widget elementor-widget-text-editor\" data-id=\"8810b05\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Rua de J\u00falio Dinis 228, 4050-371 Porto, Portugal<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>ATI2 &#8211; Preparation and functional evaluation of collagen oligopeptide-rich hydrolysates Objectives O3 &#8211; Obtain collagen oligopeptides-rich extracted from P. physalis using cold-adapted proteases from psychrophiles bacteria isolated from deep-sea. O4 \u2013 Characterization of the collagen oligopeptides-rich in terms of potential to be used in medic or dermo cosmetic applications. ATI2 This activity has two main [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_mi_skip_tracking":false,"footnotes":""},"class_list":["post-1527","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1527","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1527"}],"version-history":[{"count":19,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1527\/revisions"}],"predecessor-version":[{"id":1665,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1527\/revisions\/1665"}],"wp:attachment":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1527"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}