{"id":1552,"date":"2022-12-15T14:00:12","date_gmt":"2022-12-15T14:00:12","guid":{"rendered":"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1552"},"modified":"2022-12-16T17:40:33","modified_gmt":"2022-12-16T17:40:33","slug":"ati4","status":"publish","type":"page","link":"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1552","title":{"rendered":"ATI4"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1552\" class=\"elementor elementor-1552\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a60c1ce elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"a60c1ce\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c0a5566\" data-id=\"c0a5566\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d29922a elementor-widget elementor-widget-heading\" data-id=\"d29922a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.0 - 06-12-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">ATI4 - Assessment of the Physalia physalis toxins with potential for cosmetics and health<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a079a54 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"a079a54\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d3fe95b\" data-id=\"d3fe95b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a7f74d0 elementor-widget elementor-widget-heading\" data-id=\"a7f74d0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Objectives<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3119c5a elementor-widget elementor-widget-text-editor\" data-id=\"3119c5a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.0 - 06-12-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><em><strong>O6<\/strong> <\/em>&#8211; Identification of venom proteins with biotechnological relevance in cosmetic and therapeutic applications using transcriptomics and proteomics coupled with bioinformatics.<\/p><p><em><strong>O7<\/strong> <\/em>\u2013 Production of a library of toxin peptides.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bfa2f6e elementor-widget elementor-widget-heading\" data-id=\"bfa2f6e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI4<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b5a4ed elementor-widget elementor-widget-text-editor\" data-id=\"7b5a4ed\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Venom toxins will be identified by genomics through transcriptomic deep sequencing from P. physalis tentacle tissues to obtain a catalogue of transcripts encoding toxins.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-90626f9 elementor-widget elementor-widget-heading\" data-id=\"90626f9\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 4.1 - Identification of venom toxins by genomics<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-96fbcc0 elementor-widget elementor-widget-text-editor\" data-id=\"96fbcc0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"font-size: 16px;\">Venom characterization will be studied at genomic level. The nonexistence of P. physalis toxins data available in NCBI database hinders the correct identification of toxins. Deep sequencing technologies will be employed to generate transcriptomic sequences from tentacle tissues to obtain a catalogue of transcripts encoding toxins. In silico analysis approach will allow to search gene homology and motifs and the identification of sequences encoding the toxins, which is crucial for the identification of protein structure. These structures will be used to perform structure-based target prediction (using docking analyses) and\/ or ligand-based target prediction (by homology). The bioinformatics screening approaches will be critical for enhancing our knowledge on the complexity of venoms and their potential therapeutic. A number of specifically focused databases available, including potassium channel toxins (Kalium database), spiders (Arachnoserver), cone snails (ConoServer) and snakes venom database (ISOB), will be essential for the characterization of genes coding for relevant toxins. The data produced in this task will support the identification of venom proteins in task 4.2. This task will be performed by UA\u00c7.<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fa98cd4 elementor-widget elementor-widget-heading\" data-id=\"fa98cd4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 4.2 - Identification of venom toxins by proteomic<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0bac872 elementor-widget elementor-widget-text-editor\" data-id=\"0bac872\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Proteomic identification of venom proteins\/peptides will provide a clear understanding of repertoire of molecules with biological and biotechnological interest. Cnidaria toxins have been identified and comprise mainly proteins and peptides that are cytolytic or neurotoxic activities, includes 3.5\u20136.5 kDa voltage-gated sodium (Nav) channels toxins, 3\u20135 kDa voltage-gated potassium (Kv) channel toxins and ~20 kDa pore- forming toxins. The direct study of the jellyfish venom requires several common steps. Here will be used a nano LC-MS\/MS and MALDI-ToF\/ToF to study the venom constitutive proteins. For better quality results, the liquid chromatography LC-MS or HPLC may be used. The combination of data obtained in T4.1 and T4.2 will allow to stablish the venom profile of Physalis. Moreover, the identification of venom proteins in this task will permit to identify the presence of relevant toxins highlighted by bioinformatic analysis and to obtain paramount information for protein purification in task 4.3. This task will be performed by UA\u00c7.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1351908 elementor-widget elementor-widget-heading\" data-id=\"1351908\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 4.3 - Purification of toxins with putative activity<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c92e465 elementor-widget elementor-widget-text-editor\" data-id=\"c92e465\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>It is intended to separate the proteins\/peptides predicted with biological activities in previous tasks. To perform this separation, tangential flow filtration with different cut-off membranes, combined with size exclusion, reversed-phase chromatography and electrophoresis, will be used. We will focus specially on peptides with less than 10 kDa, which will be enriched using preparative reversed phase chromatography. Almost purified material will be tested for their biological activity in T4.4. This task will be developed by UA\u00c7.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-959dd5d elementor-widget elementor-widget-heading\" data-id=\"959dd5d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">ATI 4.4 - Characterization of relevant toxins<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9a02680 elementor-widget elementor-widget-text-editor\" data-id=\"9a02680\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The mode of action of purified toxins will be studied using Drosophila WT and Drosophila mutants (e.g., VGCCs mutant) using an ethoscope combined with a remote learning algorithm to track the profiling of behaviour in real-time. Annotation labels include the circadian rhythms, the walking distance and velocity and immobilization phenotypes. Drosophila melanogaster is a powerful molecular-genetic \u201ctoolset\u201d for the study neurotransmitter transporters and will be used here as a model to study toxin fractions. Butyrylcholinestrasic (BuChE) and acetylcholinesterase (AChE) inhibitory activities will be measured by spectrophotometric methods. The safety of active fractions will be tested measuring cytotoxicity in different cell lines (e.g., HeLa, Vero and SY5Y) and the haemolytic activity using erythrocytes. In this activity we expect to identify new active molecules with new potential applications. This task will be developed by the UA\u00c7 with the collaboration of UM in the cytotoxicity assays.<br><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-00a502d elementor-widget elementor-widget-heading\" data-id=\"00a502d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Participants<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4d80c56 elementor-widget elementor-widget-text-editor\" data-id=\"4d80c56\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><span style=\"font-size: 16px;\">Mesosystem S.A.<\/span><\/li><li><span style=\"font-size: 16px;\">Universidade do Minho<\/span><\/li><li><span style=\"font-size: 16px;\">UA\u00e7<\/span><\/li><li><span style=\"font-size: 16px;\">Nofima<\/span><\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8287b46 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"8287b46\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-af4bafc\" data-id=\"af4bafc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4b0a8c3 wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"4b0a8c3\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1436\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI1<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-9c633b8\" data-id=\"9c633b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5b9f15e wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"5b9f15e\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1527\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI2<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-f701584\" data-id=\"f701584\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f9dcf7e wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"f9dcf7e\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1538\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI3<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-8b3c83a\" data-id=\"8b3c83a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-18dcd08 wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"18dcd08\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1560\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI5<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-14caf53\" data-id=\"14caf53\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d077bd wpr-button-icon-style-inline wpr-button-icon-position-right elementor-widget elementor-widget-wpr-button\" data-id=\"3d077bd\" data-element_type=\"widget\" data-widget_type=\"wpr-button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\n\t\t\t\n\t\t\n\t\t<div class=\"wpr-button-wrap elementor-clearfix\">\n\t\t<a class=\"wpr-button wpr-button-effect wpr-button-none\" data-text=\"Go\" href=\"https:\/\/physaliaphysalis.mesosystem.com\/?page_id=1568\">\n\t\t\t\n\t\t\t<span class=\"wpr-button-content\">\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-text\">View ATI6<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c3b5737 elementor-section-height-min-height elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-items-middle wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"c3b5737\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-137aa967 elementor-hidden-tablet elementor-hidden-mobile\" data-id=\"137aa967\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-abd5fbc elementor-widget elementor-widget-heading\" data-id=\"abd5fbc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Join our mailing 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class=\"wpr-button-text\">Subscribe now<\/span>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t<span class=\"wpr-button-icon\"><i class=\"fas fa-angle-right\"><\/i><\/span>\n\t\t\t\t\t\t\t<\/span>\n\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\n\t\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e9a91f9 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"e9a91f9\" data-element_type=\"section\" id=\"contact-us\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-13a6ebb0\" data-id=\"13a6ebb0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap 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*\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-bottom:0;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right 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100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b99548c elementor-widget elementor-widget-text-editor\" data-id=\"7b99548c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>More important than what we have to say about ourselves, is what the customers have to say about us.<\/p><p>So let us learn and contact us!<\/p><p>We create possibilites for the connnected world &#8211; Mesosystem S.A.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-5813cd5a\" data-id=\"5813cd5a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-17174383 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-id=\"17174383\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-af1903c\" data-id=\"af1903c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7e2d7be1 elementor-widget elementor-widget-heading\" data-id=\"7e2d7be1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Email<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3691c7df elementor-widget elementor-widget-text-editor\" data-id=\"3691c7df\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>info@mesosystem.com<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2c008cb5 elementor-widget elementor-widget-heading\" data-id=\"2c008cb5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Phone<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-64d1386e elementor-widget elementor-widget-text-editor\" data-id=\"64d1386e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Telephone (+351) 227 347 125 (Call to a national fixed network)<\/p><p>Mobile (+351) 924 188 704 (Call to a national mobile network)<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a9afaba elementor-widget elementor-widget-heading\" data-id=\"6a9afaba\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Location<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8810b05 elementor-widget elementor-widget-text-editor\" data-id=\"8810b05\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Rua de J\u00falio Dinis 228, 4050-371 Porto, Portugal<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>ATI4 &#8211; Assessment of the Physalia physalis toxins with potential for cosmetics and health Objectives O6 &#8211; Identification of venom proteins with biotechnological relevance in cosmetic and therapeutic applications using transcriptomics and proteomics coupled with bioinformatics. O7 \u2013 Production of a library of toxin peptides. ATI4 Venom toxins will be identified by genomics through transcriptomic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_mi_skip_tracking":false,"footnotes":""},"class_list":["post-1552","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1552"}],"version-history":[{"count":10,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1552\/revisions"}],"predecessor-version":[{"id":1691,"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=\/wp\/v2\/pages\/1552\/revisions\/1691"}],"wp:attachment":[{"href":"https:\/\/physaliaphysalis.mesosystem.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}